CHEM-E8110 - Laboratory Course in Biosystems and Biomaterials Engineering, Lecture, 26.9.2023-1.12.2023
Kurssiasetusten perusteella kurssi on päättynyt 01.12.2023 Etsi kursseja: CHEM-E8110
Osion kuvaus
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Site for pre-assignments and returning reports.
Learning outcomes of pre-assignments (Part I and Part III)
- Able to recognize and understand basic terms, methods and concepts related to biolaboratory.
- Able to apply the knowledge from pre-assignment into week’s experiments.
- Able to analyze experimental data from week’s experiments in basic level, demonstrating an understanding of key concepts and principles.
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Part I Kansio
Below you will find the pre-assignments for Part I.
Any questions regarding to Part I pre-assignments: Contact Rahul Mangayil or Samuel Girmay (samuel.girmay@aalto.fi).
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Part II: Pre-assignment for BioBricks experiments Tehtävä
Questions need to be answered in written form, write 200 words
Pre-assignment is done individually
In part II you'll assemble a plasmid for expression of the fluorescent reporter protein GFP. GFP expression is often used as a proxy to measure the promoter activity. Apart from fluorescent reporter proteins, what other kind of experimental techniques can you use for testing of promoter activities? Which are the most direct/indirect methods to determine the activity of a promoter?
In these experiments you will isolate plasmid DNA from E. coli cells. Describe a method, how plasmid DNA can be isolated from E. coli. Provide as many details as are necessary for understanding how the method functions.
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Complete the feedback questionnaire related to virtual laboratories and assessment methods to further improve our virtual laboratories, assessment methods and the course design.
As I explained in kick-off lecture, feedback will be used in a study to improve virtual laboratories, assessment methods, the course as well the master's programme which is currently going a renewal for next years students. So your feedback is highly important! Feedback will be anonymous and takes about 10-15 minutes.
Also, if you answer the feedback questionnaire before the last session (1.12), you will receive a lunch ticket to Mau-kas restaurant (value 11,50 EUR). In the last session, show me that you have answered feedback and I will give you the lunch ticket. If you have any questions related to study, you can contact me for more details.
- Able to recognize and understand basic terms, methods and concepts related to biolaboratory.