CHEM-E8110 - Laboratory Course in Biosystems and Biomaterials Engineering, Lecture, 26.9.2023-1.12.2023
This course space end date is set to 01.12.2023 Search Courses: CHEM-E8110
Översikt
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Site for pre-assignments and returning reports.
Learning outcomes of pre-assignments (Part I and Part III)
- Able to recognize and understand basic terms, methods and concepts related to biolaboratory.
- Able to apply the knowledge from pre-assignment into week’s experiments.
- Able to analyze experimental data from week’s experiments in basic level, demonstrating an understanding of key concepts and principles.
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Part I Mapp
Below you will find the pre-assignments for Part I.
Any questions regarding to Part I pre-assignments: Contact Rahul Mangayil or Samuel Girmay (samuel.girmay@aalto.fi).
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Part II: Pre-assignment for BioBricks experiments Inlämningsuppgift
Questions need to be answered in written form, write 200 words
Pre-assignment is done individually
In part II you'll assemble a plasmid for expression of the fluorescent reporter protein GFP. GFP expression is often used as a proxy to measure the promoter activity. Apart from fluorescent reporter proteins, what other kind of experimental techniques can you use for testing of promoter activities? Which are the most direct/indirect methods to determine the activity of a promoter?
In these experiments you will isolate plasmid DNA from E. coli cells. Describe a method, how plasmid DNA can be isolated from E. coli. Provide as many details as are necessary for understanding how the method functions.
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Students mustFå ett betyg
Complete the feedback questionnaire related to virtual laboratories and assessment methods to further improve our virtual laboratories, assessment methods and the course design.
As I explained in kick-off lecture, feedback will be used in a study to improve virtual laboratories, assessment methods, the course as well the master's programme which is currently going a renewal for next years students. So your feedback is highly important! Feedback will be anonymous and takes about 10-15 minutes.
Also, if you answer the feedback questionnaire before the last session (1.12), you will receive a lunch ticket to Mau-kas restaurant (value 11,50 EUR). In the last session, show me that you have answered feedback and I will give you the lunch ticket. If you have any questions related to study, you can contact me for more details.
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Part I Mapp
Below you will find the pre-assignments for Part I.
Any questions regarding to Part I pre-assignments: Contact Rahul Mangayil or Samuel Girmay (samuel.girmay@aalto.fi).
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Part I: Pre-assignment (1/2): Introduction to basic biolaboratory practices and acid phosphotase assay Kunskapstest
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory, you will learn basic practices of biolaboratory and familiarize yourself with week's experiment: Acid phosphatase assay.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab1/360/
2. Once you completed the virtual biolaboratory training, complete following assessment:
Quiz assessment: Answer correctly in all the questions in the quiz.
You can keep virtual laboratory open while doing the assessment.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish).
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Part I: Pre-assignment (1/2): Introduction to basic biolaboratory practices and acid phosphotase assay Kunskapstest
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory, you will learn basic practices of biolaboratory and familiarize yourself with week's experiment: Acid phosphatase assay.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab1/360/
2. Once you completed the virtual biolaboratory training, complete following assessment:
Short-text assessment: Answer the following questions (one to five sentence per question)
- You can keep virtual laboratory open while doing the assessment.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish). - You can keep virtual laboratory open while doing the assessment.
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Part I: Pre-assignment (2/2): Basic cell culturing practices and week's experiments: Yeast crossing & Natural transformation of bacteria Kunskapstest
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory, you will learn basic practices related to cell culturing (such inoculation and streaking) and familiarize yourself with week's experiments: Yeast crossing & Natural transformation in Bacillus subtilis.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab2/360/
2. Once you completed the virtual biolaboratory training, complete following assessment:
Quiz assessment: Answer correctly in all the questions in the quiz.
You can keep virtual laboratory open while doing it assessment.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish). -
Part I: Pre-assignment (2/2): Basic cell culturing practices and week's experiments: Yeast crossing & Natural transformation of bacteria Kunskapstest
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory, you will learn basic practices related to cell culturing (such inoculation and streaking) and familiarize yourself with week's experiments: Yeast crossing & Natural transformation in Bacillus subtilis.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab2/360/
2. Once you completed the virtual biolaboratory training, complete following assessment:
Short-text assessment: Answer the following questions (one to five sentence per question).
You can keep virtual laboratory open while doing it assessment.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish). -
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Part II: Pre-assignment for BioBricks experiments Inlämningsuppgift
Questions need to be answered in written form, write 200 words
Pre-assignment is done individually
In part II you'll assemble a plasmid for expression of the fluorescent reporter protein GFP. GFP expression is often used as a proxy to measure the promoter activity. Apart from fluorescent reporter proteins, what other kind of experimental techniques can you use for testing of promoter activities? Which are the most direct/indirect methods to determine the activity of a promoter?
In these experiments you will isolate plasmid DNA from E. coli cells. Describe a method, how plasmid DNA can be isolated from E. coli. Provide as many details as are necessary for understanding how the method functions.
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Part III: Pre-assignment: Western blot Kunskapstest
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory tour, you will learn about Western blot analysis.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab3/360/
2. Once you completed the virtual biolaboratory training, complete following assessment:
''Peer-review'' assessment:
- Read the question in assignment (found when you open the quiz) and think which key points you would consider to be important in the answer. You can, for example, write them down shortly or make a
simple mind map if that helps.
- Read the answer (found when you open the quiz) from a fictional student.
- In the quiz text box, write feedback to the student. Identify all
inaccuracies, mistakes, or possible missing parts in the student's
answer. Explain why each error is incorrect and provide the correct
answers.
- Reflect on the fictional student's answer in relation to the key points you identified. Were there any similarities or differences between your initial key points and the student's response? You don't need/have to
write them in the quiz text box, this is for your own learning process.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.
If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish). - Read the question in assignment (found when you open the quiz) and think which key points you would consider to be important in the answer. You can, for example, write them down shortly or make a
simple mind map if that helps.
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Part III: Pre-assignment: ELISA Panopto Student Submission
Welcome to study safe and proper practices in biolaboratory.
In this virtual laboratory tour, you will learn about ELISA assay analysis.
Follow the steps in order to complete the assignment.
1. Go and complete the virtual biolaboratory -> https://lab.aalto.fi/biocourse2023/biolab4/360/
2. Once you completed the virtual biolaboratory training, Answer the following questions either by voice recording or video recording:
You can use Panopto to make recording (Video instruction can be found here):
(Click Add Panopto submission>Record>Capture(Browser))
OR
You can use your own recording software and upload it in this assigment
(Click Add Panopto submission>Upload)
Assignment is done individually
Describe the basic principle of ELISA assay, what are main steps and what is the purpose of these steps. During an ELISA experiment, you observe unexpectedly high background signal in your negative control wells. Propose a possible reasoning for this.
If you only want to revisit the virtual laboratory and have already done the training, you can click here (version of the virtual laboratory, where all activities and rooms are immediately available (in the bottom menu)) This version can be helpful if you have already studied the learning materials.
If you encounter technical issues with the virtual laboratory, please try first using a different web browser. If the problems persist, you can ask for help by sending email to aaltolab@aalto.fi (in English or in Finnish). -
Students mustFå ett betyg
Complete the feedback questionnaire related to virtual laboratories and assessment methods to further improve our virtual laboratories, assessment methods and the course design.
As I explained in kick-off lecture, feedback will be used in a study to improve virtual laboratories, assessment methods, the course as well the master's programme which is currently going a renewal for next years students. So your feedback is highly important! Feedback will be anonymous and takes about 10-15 minutes.
Also, if you answer the feedback questionnaire before the last session (1.12), you will receive a lunch ticket to Mau-kas restaurant (value 11,50 EUR). In the last session, show me that you have answered feedback and I will give you the lunch ticket. If you have any questions related to study, you can contact me for more details.
- Able to recognize and understand basic terms, methods and concepts related to biolaboratory.