Topic outline

  • General

    Welcome to Biolab III laboratory course!

    Please go through the most updated version of Aalto corona instructions:

    https://www.aalto.fi/en/aalto-university/internal-instructions-related-to-coronavirus

    The lab work will take place in C305. On the first lab day we will get you from the Chemistry building main lobby if you don't know that location.

    The course comprises two independent projects. In both projects the participants will mainly work in pairs. In addition, there will be reporting, assignments and presentations/discussions during the course. As we have a capacity limit in the teaching laboratory we might have to divide the class into two working groups (see preliminary scheduling on this page).

    In the first project you will use baker's yeast for expression of a therapeutic protein. Within the three to four first weeks you will cover the whole work flow - from generating new yeast strains to analyzing the final strains. The work in this project will be done according to protocols and predefined schedule.

    In the second project, you will use BioBricks to build expression constructs in E. coli. The project will be planned and conducted more freely in pairs within the given result requirements and time frames.

    Please be aware that during corona times we must follow the Aalto level restrictions and instructions so changes to course schedule and content might take place.


    • File icon
      Preliminary weekly schedule, yeast work File
      PDF document
      Not available unless: Your Email address contains @aalto.fi

      Depending on the number of enrolled students you might be divided to two working groups in the beginning of the course. Here is a preliminary weekly schedule for the first four weeks of the course after which you will continue with the E. coli work with more free scheduling.

    • Group choice icon
      Choose the laboratory working group, project 1 (yeast work) Group choice
      Not available unless any of:
      • You are a(n) Advanced teacher
      • You are a(n) Teacher
      • You are a(n) Student

      Check the preliminary timetable for project 1 of the course and choose your preferred lab group. If you want to work with a specific partner you both have to select the same group.

    • File icon
      Laboratory manual, yeast part File
      Word 2007 document
      Not available unless any of:
      • You are a(n) Student
      • You are a(n) Teacher
      • You are a(n) Advanced teacher

      The lab manual contains:

      • General background information of the yeast project
      • Introduction of the experiment
      • Short description of daily work
      • Detailed protocols
      • Collection of recipes for some media and buffers
      You will get the laboratory manual in a printed form on the first lab day.

    • Group choice icon
      Choose the working mode for project 2 (weeks 13-14) Group choice
      Not available unless any of:
      • You are a(n) Student
      • You are a(n) Teacher
      • You are a(n) Advanced teacher
    • File icon
      Remote and lab work File
      Word 2007 document
      Not available unless any of:
      • You are a(n) Student
      • You are a(n) Teacher
      • You are a(n) Advanced teacher
    • Scheduler icon
      Laboratory work on weeks 13 and 14 Scheduler
    • Forum icon
      General discussion Forum
    • Forum icon
      Announcements Forum
    • Assignment icon
      Self and group assessment Assignment
      Not available unless: You are a(n) Student

      Please fill in this short assessment file and submit it in MyCourses. You can answer in English or in Finnish. This is an individual assessment so do it on your own, not with your pair.

      Naturally, this assessment will not be graded but you will get your final grade only if you have returned the assessment file.


    • File icon
      Course grades File
      PDF document
      Not available unless: You are a(n) Student