Topic outline

  • Materials

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      Course start meeting File
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      Instructions to teaching lab File
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      Lecture 16.3. and 17.3.2021 File
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      Lab manual for E. coli project File
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      Lab work start session 23.3. File
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      Remote work start session 23.3. File
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      Group 5-7 immunoblot (WB) and ELISA results Folder
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      Remote work mode: E. coli cloning process File
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      In silico cloning exercise Folder
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      If you want to practice in silico cloning, first watch the DNA Atlas tutorial video from this same page and then try to digest and ligate the constitutive promoter plasmid with the GFP cassette in DNA Atlas. Some of you have already done this in Biolab I, but you can still do this exercise if you like. If you need more challenge, you can search different plasmids from the iGEM resources, digest and ligate them and create your own novel in silico recombinant plasmids. There are also the common BioBrick primer sequences provided, the same that you are using in colony PCR.

      If you want to have some feedback on the exercise during the course or later in period V, just contact Heli.

      Please remember that this exercise is not mandatory, it is for you to learn and practice the in silico cloning process.

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      Colony PCR gel results File
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      Colony PCR products gel File
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      Colony PCR products File
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      Lab groups, Cytation data handling File
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      Cytation results 07.04.21 File
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      Cytation results 08.04.21 File
      Excel 2007 spreadsheet
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