CHEM-E3160 - Biolab III, 01.03.2021-20.04.2021
This course space end date is set to 20.04.2021 Search Courses: CHEM-E3160
Översikt
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Course start meeting FilPDF-dokument
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Instructions to teaching lab FilPDF-dokument
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Lecture 16.3. and 17.3.2021 FilPDF-dokument
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Lab manual for E. coli project FilPDF-dokument
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Lab work start session 23.3. FilPDF-dokument
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Remote work start session 23.3. FilPDF-dokument
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Group 5-7 immunoblot (WB) and ELISA results Mapp
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Remote work mode: E. coli cloning process FilWord 2007-dokument
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In silico cloning exercise Mapp
If you want to practice in silico cloning, first watch the DNA Atlas tutorial video from this same page and then try to digest and ligate the constitutive promoter plasmid with the GFP cassette in DNA Atlas. Some of you have already done this in Biolab I, but you can still do this exercise if you like. If you need more challenge, you can search different plasmids from the iGEM resources, digest and ligate them and create your own novel in silico recombinant plasmids. There are also the common BioBrick primer sequences provided, the same that you are using in colony PCR.
If you want to have some feedback on the exercise during the course or later in period V, just contact Heli.
Please remember that this exercise is not mandatory, it is for you to learn and practice the in silico cloning process.
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Lab groups, Cytation data handling FilWord 2007-dokument
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